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The density of α smooth muscle actin, desmin, and vimentin, (right curve) within cells derived from granulation tissue of wounds compared with an isotypic control (curve in the middle) and untreated cells of control (left curve) shown by flow cytometry profiles. The figures represent untreated cells (Fig. 1a), cells treated with histamine (Fig. 1b), ketotifen (Fig.1c), ranitidine (Fig. 1d), cultures treated with histamine and ketotifen (Fig.1e) as well as pyridylethylamine dihydrochloride (Fig. 1f). FITC-A represents the fluorescence intensity of fluorescein isothiocyanate (FITC) complexed with antibodies

Journal: Molecular and Cellular Biochemistry

Article Title: Histamine augments collagen content via H1 receptor stimulation in cultures of myofibroblasts taken from wound granulation tissue

doi: 10.1007/s11010-020-03974-6

Figure Lengend Snippet: The density of α smooth muscle actin, desmin, and vimentin, (right curve) within cells derived from granulation tissue of wounds compared with an isotypic control (curve in the middle) and untreated cells of control (left curve) shown by flow cytometry profiles. The figures represent untreated cells (Fig. 1a), cells treated with histamine (Fig. 1b), ketotifen (Fig.1c), ranitidine (Fig. 1d), cultures treated with histamine and ketotifen (Fig.1e) as well as pyridylethylamine dihydrochloride (Fig. 1f). FITC-A represents the fluorescence intensity of fluorescein isothiocyanate (FITC) complexed with antibodies

Article Snippet: The following antibodies were applied for the staining: Vimentin LN-6, (Novus Biologicals, Littleton, CO, USA), Mouse IgM Isotype Control, (eBioscience San Diego, USA), Desmin DES/1711, (Novus Biologicals, Littleton, CO, USA), Mouse IgG1 Kappa Light Chain Isotype Control, (Novus Biologicals, Littleton, CO, USA), Alpha- Smooth Muscle Actin (Novus Biologicals, Littleton, CO, USA), and Mouse IgG2a Kappa Light Chain Isotype Control (Novus Biologicals, Littleton, CO, USA).

Techniques: Derivative Assay, Flow Cytometry, Fluorescence

The density of α smooth muscle actin, desmin, and vimentin, (right curve) within cells derived from granulation tissue of wounds compared with an isotypic control (curve in the middle) and untreated control cells (left curve) shown by flow cytometry profiles. FITC-A represents the fluorescence intensity of fluorescein isothiocyanate (FITC) complexed with antibodies (Fig. 3a). Collagen content (μg/10 5 cells) within myofibroblast cultures isolated from intact skin, in the control group (CTR) and in cells treated with histamine at concentrations of 10 −4 M (Hi10-4), 10 −5 M (Hi10-5), and 10 −6 M (Hi10-6). Each value expresses the mean of ten cultures + standard deviation (SD), (Fig. 3b)

Journal: Molecular and Cellular Biochemistry

Article Title: Histamine augments collagen content via H1 receptor stimulation in cultures of myofibroblasts taken from wound granulation tissue

doi: 10.1007/s11010-020-03974-6

Figure Lengend Snippet: The density of α smooth muscle actin, desmin, and vimentin, (right curve) within cells derived from granulation tissue of wounds compared with an isotypic control (curve in the middle) and untreated control cells (left curve) shown by flow cytometry profiles. FITC-A represents the fluorescence intensity of fluorescein isothiocyanate (FITC) complexed with antibodies (Fig. 3a). Collagen content (μg/10 5 cells) within myofibroblast cultures isolated from intact skin, in the control group (CTR) and in cells treated with histamine at concentrations of 10 −4 M (Hi10-4), 10 −5 M (Hi10-5), and 10 −6 M (Hi10-6). Each value expresses the mean of ten cultures + standard deviation (SD), (Fig. 3b)

Article Snippet: The following antibodies were applied for the staining: Vimentin LN-6, (Novus Biologicals, Littleton, CO, USA), Mouse IgM Isotype Control, (eBioscience San Diego, USA), Desmin DES/1711, (Novus Biologicals, Littleton, CO, USA), Mouse IgG1 Kappa Light Chain Isotype Control, (Novus Biologicals, Littleton, CO, USA), Alpha- Smooth Muscle Actin (Novus Biologicals, Littleton, CO, USA), and Mouse IgG2a Kappa Light Chain Isotype Control (Novus Biologicals, Littleton, CO, USA).

Techniques: Derivative Assay, Flow Cytometry, Fluorescence, Isolation, Standard Deviation